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FCUP

PrimerIdent

	PrimerIdent
	Introduction
	Application Example
	F.A.Q.
	Citation and Contacts

Introduction

Background

PrimerIdent is a web-based primer design tool conceived to help designing DNA oligonucleotide primers suitable for:

[››]amplification of DNA sequences from understudied plant species or other organisms (conserved primers);

[››]effective specific distinction of cDNA sequences from the same organism that share great identity among members of the same gene family.

Conserved primers across multiple species and simultaneously specific for a certain isozyme can be rare and difficult to find. PrimerIdent was developed aiming to automate this primer design and selection process in a given nucleotide sequences alignment, providing an intuitive, easy to interpret graphical result, which provides a list of all possible primers that meet the user criteria, with a colour-code identity to each sequence in the alignment.

PrimerIdent Introduction by Alberto Pessoa, Susana Pereira and Jorge Teixeira is licensed under a
Creative Commons Attribution 2.5 License.

Copyright Notice
PrimerIdent is a Copyright (c) of Alberto Pessoa, Faculdade de Ciências - Universidade do Porto, Portugal; 2009-2013. PrimerIdent uses Primer3, Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008,2009,2010,2011,2012 Whitehead Institute for Biomedical Research, Steve Rozen, and Helen Skaletsky All rights reserved.